Thermo Scientific™ Verso 1-Step RT-PCR Kits

Descrizione

Verso 1-Step RT-PCR system is a complete set of RT-PCR reagents that combines everything needed for RT-PCR. The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures. Verso also shows a better dynamic range than standard AMV or M-MuLV enzymes, with increases in template concentration giving rise to corresponding increases in product generated over a wider range of starting material concentrations.

The Verso system is available with ThermoPrime (standard PCR) or Thermo-Start (hot start) polymerase, the latter providing a more specific option that delivers higher yields and greatly reduces nonspecific primer extension before PCR. Thermo-Start Taq DNA Polymerase is a chemically-modified polymerase that remains inactive throughout the RT step, activated only by the high-temperature incubation (95°C for 15 minutes) prior to PCR.

An optional RT Enhancer is provided with kits and can be used during the RT step to remove any DNA contamination, eliminating the need for DNase I treatment.

The Verso 1-step RT-PCR system is also available with a ReddyMix option, where the master mix contains a dye and precipitant that allow RT-PCR reactions to be loaded directly onto an electrophoresis gel. The red dye migrates between bromophenol blue and xylene cyanol at approximately 300 bp, depending on agarose concentration.

• Reverse transcription and amplification steps are carried out in a single reaction tube delivering convenient, fast analysis
• Highly sensitive with a broad dynamic range: can accurately detect RNA input from 1pg to 1μg
• High-affinity RT enzyme effectively transcribes through difficult RNA sequences
• Optimized 1-Step buffer maximizes performance from both RT and PCR steps
• Wide working temperature range (42° to 57°C) for success with GC-rich and other difficult templates
• Included RT Enhancer prevents genomic DNA carryover, eliminating the need for separate DNase I treatment
• RNase Inhibitor included in RT enzyme mix

Format Options:

1-Step RT-PCR Master Mix w/ThermoPrime Taq

• Contains ThermoPrime Taq DNA polymerase for highly sensitive detection and analysis of RNA

1-Step RT-PCR ReddyMix Master Mix w/ThermoPrime Taq

• Contains ThermoPrime Taq DNA polymerase for highly sensitive detection and analysis of RNA
• ReddyMix Master Mix contains an inert red tracking dye and gel-loading precipitant
• Reaction mix can be directly loaded onto agarose gels post-PCR, without the addition of loading dye
• Red dye allows for easy sample visualization during reaction setup

1-Step RT-PCR Master Mix w/Thermo-Start Taq (Hot Start)

• Contains Thermo-Start Taq DNA polymerase for hot start PCR activation to deliver maximum sensitivity and specificity
• Safe for room temperature reaction setup
• Thermo-Start Taq remains completely inactive until it is switched on by a high temperature incubation step at the start of the PCR reaction
• Hot-start technology prevents non-specific amplification during reaction setup and the RT step, eliminating smearing and non-target bands

Verso RNA Control Kit

• Intended for use as a positive control to monitor the performance of all RT-PCR components
• Kit includes a 3.6kb RNA synthesized according to the sequence derived from bacteriophage MS2 and primers designed to generate a 522 bp product

Compatible with:

Verso cDNA Synthesis Kits

Recommended for:

1-Step RT-PCR