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IBA Lifesciences Strep-Tactin™ Conjugate
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Product Type:
AP Conjugate
Coniugato Strep-Tactin-HRP
Dimensione della confezione:
500µL
Pezzo
Descrizione
- The streptavidin variant Strep-Tactin™ is available as conjugate with horseradish peroxidase (HRP) or with alkaline phosphatase (AP) for fast and directed detection of N-terminally, C-terminally, or internally tagged Strep-tag™II or Twin-Strep-tag™ fusion proteins transferred to a blotting membrane or captured on a microplate.
- Both conjugates are suitable for chromogenic reaction, whereas Strep-Tactin™ HRP is applicable for chemiluminescence reaction as well.
- Depending on the host organism, protein extracts may contain small amounts of biotinylated proteins, e.g., the biotin carboxyl carrier protein (BCCP, 22 kDa) of E. coli.
- Biotin Blocking Buffer is recommended for blocking of such biotinylated proteins on western blots prior to detection of Strep-tag™II or Twin-Strep-tag™ fusion proteins with Strep-Tactin™ HRP to prevent the detection of false positive bands.
- For this purpose, Biotin Blocking Buffer contains the tetrameric biotin binding protein avidin, which selectively binds biotinylated proteins.
Specifica
Specifica
Quantità | 0.5 mL |
Content And Storage | 4 °C |
Product Type | AP Conjugate |
Esclusivamente per scopi di ricerca.
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