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  5. IBA Lifesciences StarGate pPSG-IBA Acceptor Vectors for E. coli Expression

IBA Lifesciences StarGate pPSG-IBA Acceptor Vectors for E. coli Expression

Achieves high-level expression in E. coli by the T7 promoter by providing specified genetic surroundings for optimal expression and purification of non-toxic proteins.

220.00€

Specifica

Formato Liquid
Da utilizzare con (apparecchiatura) StarGate™ System, E. Coli BL21(DE3)
Agente inducente E. coli host with T7 RNA polymerase gene (e.g. BL21(DE3))
pH 8
Promotore T7
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Prodotti 22
Codice del prodotto Marca Vettore Prezzo Quantità e disponibilità  
Codice del prodotto Marca Vettore Prezzo Quantità e disponibilità  
15633758
Vedere i documenti
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IBA Lifesciences
5-4200-001 		pPSG-IBA wt1 N/A N/A N/A
15643758
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IBA Lifesciences
5-4203-001 		pPSG-IBA 3 N/A N/A N/A
15653758
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IBA Lifesciences
5-4205-001 		pPSG-IBA 5 N/A N/A N/A
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IBA Lifesciences
5-4223-001 		pPSG-IBA 23 N/A N/A N/A
15673758
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IBA Lifesciences
5-4225-001 		pPSG-IBA 25 N/A N/A N/A
15683758
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IBA Lifesciences
5-4233-001 		pPSG-IBA 33 N/A N/A N/A
15693758
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IBA Lifesciences
5-4235-001 		pPSG-IBA35 N/A N/A N/A
15603768
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IBA Lifesciences
5-4243-001 		pPSG-IBA43 N/A N/A N/A
15613768
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IBA Lifesciences
5-4245-001 		pPSG-IBA45 N/A N/A N/A
15623768
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IBA Lifesciences
5-4262-001 		pPSG-IBA 62 N/A N/A N/A
15633768
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IBA Lifesciences
5-4263-001 		pPSG-IBA 63 N/A N/A N/A
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IBA Lifesciences
5-4264-001 		pPSG-IBA 64 N/A N/A N/A
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IBA Lifesciences
5-4265-001 		pPSG-IBA 65 N/A N/A N/A
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IBA Lifesciences
5-4303-001 		pPSG-IBA103 N/A N/A N/A
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IBA Lifesciences
5-4305-001 		pPSG-IBA 105 N/A N/A N/A
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IBA Lifesciences
5-4323-001 		pPSG-IBA 123 N/A N/A N/A
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IBA Lifesciences
5-4343-001 		pPSG-IBA143 N/A N/A N/A
15603778
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IBA Lifesciences
5-4345-001 		pPSG-IBA145 N/A N/A N/A
15613778
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IBA Lifesciences
5-4362-001 		pPSG-IBA162 N/A N/A N/A
15623778
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IBA Lifesciences
5-4364-001 		pPSG-IBA 164 N/A N/A N/A
15633778
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IBA Lifesciences
5-4367-001 		pPSG-IBA167 N/A N/A N/A
15643778
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IBA Lifesciences
5-4368-001 		pPSG-IBA 168 N/A N/A N/A
Descrizione

Descrizione

pPSG-IGA E. coli expression vectors:

pPSG-IBA vectors use the T7 promoter and T7 RNA polymerase for high-level transcription of the gene of interest.

  • As the T7 promoter is stronger than the tet promoter, pPSG-IBA vectors are recommended in cases where expression with the tet promoter does not lead to significant yields of the recombinant protein.

    • High-level expression in E. coli by bacteriophage T7 promoter
    • High-level transcription by T7 RNA polymerase in BL21 strains
    • High-level expression of non-toxic proteins
    • Induction by IPTG
    • Suitable for in vitro transcription/translation

    StarGate System

    IBA developed StarGate to provide a simple, fast and highly efficient cloning process for genes of interest (GOI) for use in functional expression, purification, detection, immobilization or separation of recombinant proteins:

    • Minimal extra modification of the gene due to short combinatorial sites
    • Inherent high-level cloning efficiency due to a directed reaction (no equilibrium)
    • GOIs are cloned to obtain a Donor Vector; 2 or more genes can be combined using Fusion Cloning
    • Genes are then transferred into Acceptor Vectors that provide optimal genetic surroundings
    • The final expression vector, the Destination Vector, is then placed into the respective host
    • Specifica

    Specifica

    Liquid
    E. coli host with T7 RNA polymerase gene (e.g. BL21(DE3))
    T7
    2°C to 8°C for frequent usage, -20°C for long-term storage
    5 μg
    Cloning genes of interest for use in functional expression, purification, detection, immobilization or separation of recombinant proteins
    StarGate™ System, E. Coli BL21(DE3)
    8
    20 μL TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5 μg
    250 ng/μL
    Clear
    Videos
    Sicurezza e movimentazione

    Sicurezza e movimentazione

    missing translation for 'shelfLife' : 12 months

    SDS
    Documenti

    Documenti

    Product Certifications
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