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IBA Lifesciences StarGate pLSG-IBA Vectors for Baculo
Transfer vectors that allow for high-level expression in insect cells by introducing the gene of interest into the Polyhedrin gene locus of AcMNPV DNA by homologous recombination.
220.00€ - 266.00€
Specifica
| Formato | Liquid |
|---|---|
| Da utilizzare con (apparecchiatura) | StarGate™ System, Insect Host Cells |
| Agente inducente | BacPAK6 linearized A cmNPV DNA (Clontech) or circular flashBAC modified A cmNPV DNA (Oxford Expression Technologies) |
| pH | 8 |
| Promotore | Polyhedrin |
| Codice del prodotto | Marca | Vettore | Prezzo | Quantità e disponibilità | |||||
|---|---|---|---|---|---|---|---|---|---|
| Codice del prodotto | Marca | Vettore | Prezzo | Quantità e disponibilità | |||||
|
15623828
|
IBA Lifesciences
5-4800-001 |
pLSG-IBA wt1 | N/A | N/A | N/A | ||||
|
15633828
|
IBA Lifesciences
5-4801-001 |
pLSG-IBA wt2 | N/A | N/A | N/A | ||||
|
15643828
|
IBA Lifesciences
5-4803-001 |
pLSG-IBA 3 | N/A | N/A | N/A | ||||
|
15653828
|
IBA Lifesciences
5-4805-001 |
pLSG-IBA 5 | N/A | N/A | N/A | ||||
|
15663828
|
IBA Lifesciences
5-4823-001 |
pLSG-IBA 23 | N/A | N/A | N/A | ||||
|
15673828
|
IBA Lifesciences
5-4825-001 |
pLSG-IBA 25 | N/A | N/A | N/A | ||||
|
15683828
|
IBA Lifesciences
5-4833-001 |
pLSG-IBA 33 | N/A | N/A | N/A | ||||
|
15693828
|
IBA Lifesciences
5-4835-001 |
pLSG-IBA 35 | N/A | N/A | N/A | ||||
|
15603838
|
IBA Lifesciences
5-4843-001 |
pLSG-IBA 43 | N/A | N/A | N/A | ||||
|
15613838
|
IBA Lifesciences
5-4845-001 |
pLSG-IBA 45 | N/A | N/A | N/A | ||||
|
15623838
|
IBA Lifesciences
5-4862-001 |
pLSG-IBA 62 | N/A | N/A | N/A | ||||
|
15633838
|
IBA Lifesciences
5-4863-001 |
pLSG-IBA 63 | N/A | N/A | N/A | ||||
|
15643838
|
IBA Lifesciences
5-4864-001 |
pLSG-IBA 64 | N/A | N/A | N/A | ||||
|
15653838
|
IBA Lifesciences
5-4865-001 |
pLSG-IBA 65 | N/A | N/A | N/A | ||||
|
15663838
|
IBA Lifesciences
5-4902-001 |
pLSG-IBA 102 | N/A | N/A | N/A | ||||
|
15673838
|
IBA Lifesciences
5-4903-001 |
pLSG-IBA 103 | N/A | N/A | N/A | ||||
|
15683838
|
IBA Lifesciences
5-4904-001 |
pLSG-IBA 104 | N/A | N/A | N/A | ||||
|
15693838
|
IBA Lifesciences
5-4905-001 |
pLSG-IBA 105 | N/A | N/A | N/A | ||||
|
15603848
|
IBA Lifesciences
5-4923-001 |
pLSG-IBA 123 | N/A | N/A | N/A | ||||
|
15613848
|
IBA Lifesciences
5-4942-001 |
pLSG-IBA 142 | N/A | N/A | N/A | ||||
|
15623848
|
IBA Lifesciences
5-4943-001 |
pLSG-IBA 143 | N/A | N/A | N/A | ||||
|
15633848
|
IBA Lifesciences
5-4944-001 |
pLSG-IBA 144 | N/A | N/A | N/A | ||||
|
15643848
|
IBA Lifesciences
5-4945-001 |
pLSG-IBA 145 | N/A | N/A | N/A | ||||
|
15653848
|
IBA Lifesciences
5-4962-001 |
pLSG-IBA 162 | N/A | N/A | N/A | ||||
|
15663848
|
IBA Lifesciences
5-4964-001 |
pLSG-IBA 164 | N/A | N/A | N/A | ||||
|
15673848
|
IBA Lifesciences
5-4967-001 |
pLSG-IBA 167 | N/A | N/A | N/A | ||||
|
15683848
|
IBA Lifesciences
5-4968-001 |
pLSG-IBA 168 | N/A | N/A | N/A | ||||
Descrizione
pLSG-IBA Baculo virus (insect cells) expression vectorsThe pLSG-IBAwt1 vector carries the Polyhedrin promoter for high-level expression in insect cells.
- Co-transfection with BacPAK6 linearized AcMNPV DNA (Clontech) or circular flashBAC modified AcMNPV DNA (Oxford Expression Technologies) allows the generation of recombinant baculovirus at very high efficiency through reconstitution of an essential gene (ORF 1629) and elimination of wild type virus to great extent.
- Cloning and expression of recombinant proteins in insect cells
- Polyhedrin promoter drives high-level expression of the insert
- Ampicillin resistance and ColE1 origin of replication (pUC) support propagation in E. coli
Please note that cloning into pLSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pLSG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.
Specifica
| Liquid | |
| BacPAK6 linearized A cmNPV DNA (Clontech) or circular flashBAC modified A cmNPV DNA (Oxford Expression Technologies) | |
| Polyhedrin | |
| 20 μL TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5 μg | |
| 250 ng/μL | |
| Clear |
| StarGate™ System, Insect Host Cells | |
| 8 | |
| Acceptor Vector for bacula (insect cell) expression | |
| 2°C to 8°C for frequent usage, -20°C for long-term storage | |
| 5 μg | |
| Cloning Genes of Interest for Use in Functional Expression, Purification, Detection, Immobilization or Separation of Recombinant Proteins |
Sicurezza e movimentazione
missing translation for 'shelfLife' : 12 months
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