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Cytiva Ready-To-Go™ T4 DNA Ligase
For covalently linking DNA fragments in as little as 30 minutes
Marca: Cytiva 27-0361-01
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Descrizione
- Catalyzes the formation of a phosphodiester bond between the 5'-phosphoryl group and the 3'-hydroxyl group of two double-stranded DNA fragments
- Consists of a glassified reaction mix predispensed in single-reaction tubes
- When reconstituted to final reaction volume of 20μL, yields solution of reaction buffer, T4 DNA ligase, and ATP
Specifica
| FPLCpure™ T4 DNA Ligase 6 weiss units, 66mM Tris HCl (pH 7.6), 6.6mM MgCl2, 0.1mM ATP, 0.1mM Spermidine, 10mM DTT and stabilizer, glassified reaction mix predispensed in single-reaction tubes | |
| TE Buffer (10mM Tris HCl, pH 8, with 1mM EDTA) or 1-2X One-phor-all Buffer | |
| 50 Reactions |
| Self-circularization of linear DNA prior to transformation, ligation of insert to vector and ligation of synthetic linkers to blunt-ended DNA | |
| Room Temperature | |
| Ready-To-Go T4 DNA Ligase, 50 reaction beads |
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