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Thermo Scientific™ Nucleasi micrococcica Pierce™

Codice prodotto. 11835135 Sfoglia Tutto Thermo Scientific Prodotti
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Quantità:
150 μL
Dimensione della confezione:
150µL
Les retours ne sont pas autorisés pour ce produit. Consulta la politica di reso
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24112” to get your promotional price
Les retours ne sont pas autorisés pour ce produit. Consulta la politica di reso
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24112” to get your promotional price

Descrizione

Remove nucleic acids from cell lysates, release chromatin-bound proteins and shear chromatin for chromatin immunoprecipitation (ChIP) using micrococcal nuclease.

Thermo Scientific™ Micrococcal Nuclease (MNase) is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments.

This micrococcal nuclease is a stable liquid form of the enzyme derived from Staphylococcus aureus. Micrococcal nuclease (MNase) exhibits exo- and endo-5'-phosphodiesterase activities against DNA and RNA. This enzyme digests double-stranded, single-stranded, circular and linear nucleic acids. The highest activity is toward single-stranded nucleic acid substrates with preference for AT- or AU-rich regions. Enzymatic activity occurs at pH 7 to 10 and is strictly dependent on calcium for the digestion of RNA and DNA substrates. Micrococcal nuclease is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments.

Highlights:

  • Digests nucleic acids (DNA and RNA)

  • Effective for double-stranded, single-stranded, circular and linear nucleic acids
  • Active in neutral to alkaline buffer conditions containing divalent calcium ions
  • Supplied at ≥100 units/μL in 10mM Tris-HCl, pH 7.5, 50mM NaCl, 1mM EDTA, 50% glycerol

Compatible with:

Thermo Scientific Subcellular Protein Fractionation Kit (Part No. 78840); Thermo Scientific Pierce ChIP Kit (Part No. 26156)

Recommended for:

Enzymatic DNA shearing for chromatin immunoprecipitation assays (ChIP); Removal of unwanted genomic or organellar nucleic acids from cell lysates; Degradation of nucleic acids in subcellular fractionation procedures

TRUSTED_SUSTAINABILITY

Specifica

Product Type Cell Lysis Enzyme
Content And Storage Upon receipt store at -20°C. Product is shipped with dry ice.
Forma Liquid
Tipo di reagente Enzyme for Cell Lysis
Quantità 150 μL

For Research Use Only. Not for use in diagnostic procedures.

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