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Thermo Scientific™ Inclusion Body Solubilization Reagent
Retrieve recombinant proteins that form insoluble aggregates during bacterial overexpression with this reagent optimized for inclusion body solubilization.
Marca: Thermo Scientific™ 78115
205.34 EUR valido fino al 2025-06-21
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Allerta:
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Descrizione
Effectively retrieve recombinant proteins that form insoluble aggregates during bacterial overexpression with Thermo Scientific™ Inclusion Body Solubilization Reagent.
The Inclusion Body Solubilization Reagent is a ready-to-use aqueous solution of denaturant that helps to dissolve aggregated proteins (inclusion bodies) that have been separated from the soluble fraction of bacterial cell lysates. The reagent is designed to retrieve expressed protein in soluble form after lysis and extraction with reagents such as Thermo Scientific B-PER Bacterial Protein Extraction Reagent. Bacteria are widely used for recombinant protein expression. However, 70%-80% of proteins produced by recombinant techniques in E. coli form inclusion bodies (i.e., protein aggregates). Once these aggregates are formed, it is very difficult to solubilize them. A proprietary denaturant contained in this reagent provides the most effective means for solubilizing aggregated proteins. Additional components, such as a reducing agent and a chelating agent, may be added to the reagent, depending on the particular application.Highlights:
- Achieves the essential first step for subsequent insoluble protein refolding procedures
- One easy- and ready-to-use solution; no preparation needed
- Compatible with Thermo Scientific Coomassie Plus Protein Assay Kit (Part No. 23236) to determine degree of expression
- Proprietary denaturant can be easily removed by dialysis so that SDS-PAGE can be performed
- Can accommodate additional components such as reducing agents or chelating agents
Compatible with:
Thermo Scientific Pierce Protein Refolding Kit (Part No. 89867)

Specifica
Solubilization Reagent | |
Liquid | |
100 mL |
Upon receipt store at room temperature. | |
Chaotrope, Denaturant |
Sicurezza e movimentazione
- Inclusion Body Solubilization Reagent
Avvertenza
- Attenzione
Categoria di pericolo
- Tossicità acuta Categoria 4
- Lesione oculare grave/irritazione oculare Categoria 2
- Corrosione/irritazione della pelle Categoria 2
Indicazioni di pericolo
- H302-Nocivo se ingerito.
- H315-Provoca irritazione cutanea.
- H319-Provoca grave irritazione oculare.
Consigli di prudenza
- P260-Non respirare la polvere/i fumi/i gas/la nebbia/i vapori/gli aerosol.
- P280-Indossare guanti/indumenti protettivi/Proteggere gli occhi/il viso.
- P301+P310-IN CASO DI INGESTIONE: contattare immediatamente un CENTRO ANTIVELENI/un medico/
- P305+P351+P338-IN CASO DI CONTATTO CON GLI OCCHI: sciacquare accuratamente per parecchi minuti. Togliere le eventuali lenti a contatto se è agevole farlo. Continuare a sciacquare.
- P501b-Smaltire il prodotto/recipiente in conformità con le disposizioni locali/regionali/nazionali/internazionali.
Informazioni supplementari
- MIXTURE LIST-Contiene: alkaline guanidine salt
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For Research Use Only. Not for use in diagnostic procedures.