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Thermo Scientific™ Kit di co-immunoprecipitazione Pierce™
Covalently immobilize IP antibodies and perform co-IP assays in which target protein complexes are immunoprecipitated without antibody contamination.
Marca: Thermo Scientific™ 26149
361.35 EUR valido fino al 2025-06-21
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Description
Thermo Scientific™ Pierce Co-Immunoprecipitation Kit provides all the components necessary to perform a properly controlled co-IP experiment and obtain purified products without antibody contamination.
The Co-Immunoprecipitation (Co-IP) Kit enables isolation of native protein complexes from a lysate or other complex mixture by directly immobilizing purified antibodies onto an agarose support. Co-IP is a common approach to study protein:protein interactions that uses an antibody to immunoprecipitate the antigen (bait protein) and co-immunoprecipitate any interacting proteins (prey proteins). Traditional co-IP methods that use Protein A or G result in co-elution of the antibody heavy and light chains that may co-migrate with relevant bands, masking important results. The Pierce Co-IP Kit resolves this issue by covalently coupling antibodies onto an amine-reactive resin. The kit includes optimized buffers for protein binding and recovery, reagents to perform control experiments and efficient spin columns and collection tubes, which shorten the protocol and minimize handling and mixing.Highlights:
Works for any purified antibody – perform co-immunoprecipitation experiments using any purified antibody regardless of species or Ig class (chicken IgY, human IgE, mouse IgG1, IgM, etc.); no dependence on Protein A and Protein G affinity binding
No antibody interference – covalent attachment method and non-reducing elution system yields pure co-IP products without contamination by the IP antibody
Reusable antibody beads – covalent antibody immobilization allows prepared IP resin to be reused and provides potential savings of costly antibody
Complete Kit – all reagents for antibody immobilization and many IP experiments, including binding and wash buffers, elution buffer and convenient microcentrifuge spin columns to make resin manipulations easier and more efficient
Control beads included – underivatized agarose beads provided for use as negative control for nonspecific binding
Versatile – co-IP method is compatible with any physiolgical (non-denaturing) protein sample buffer that is compatible with the specific antibody-antigen and protein interaction complex
Includes:
AminoLink Plus Coupling Resin, coupling buffer, quenching buffer, wash solution, sodium cyanoborohydride solution, IP lysis/wash buffer, conditioning buffer, Tris-buffered saline, elution buffer, lane marker sample buffer, control agarose resin, spin columns and collection tubes
Specifications
| Sufficient For: Preparing and performing 50 co-IP reactions, each with 25 μL of resin • AminoLink Plus Coupling Resin, 2 mL • Coupling Buffer (20X), 25 mL • Sodium Cyanoborohydride Solution (5M), 0.5 mL • Quenching Buffer, 50 mL • Wash Solution, 60 mL • IP Lysis/Wash Buffer, 2 x 50 mL • Modified Dulbecco's PBS (20X), 25 mL • Conditioning Buffer (100X), 5 mL • Elution Buffer, 50 mL • Lane Marker Sample Buffer (5X), 5 mL • Pierce Spin Columns, Screw Cap, 50 columns • Microcentrifuge Collection Tubes, 2 mL, 100 tubes • Microcentrifuge Collection Tubes, 1.5 mL, 50 tubes • Pierce Control Agarose Resin, 2 mL Store at 4°C. |
|
| Microcentrifuge | |
| 50 reactions kit | |
| Not Target-Specific | |
| 50 Reactions |
| Affinity Purification | |
| Pierce™ | |
| Immunoprecipitation (IP) | |
| Immunoprecipitation/Co-Immunoprecipitation Kit | |
| Kit |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Pierce Co-Immunoprecipitation Kit
Signal Word
- Pericolo
Hazard Category
- Liquido infiammabile Categoria 3
- Tossicità acuta Categoria 2
- Corrosione/irritazione della pelle Categoria 1B
- Lesione oculare grave/irritazione oculare Categoria 1
- Corrosione/irritazione della pelle Categoria 2
- Lesione oculare grave/irritazione oculare Categoria 2
- PERICOLO ACUTO PER L'AMBIENTE ACQUATICO Acuto 1
Hazard Statement
- H226-Liquido e vapori infiammabili.
- H300-Letale se ingerito.
- H310-Letale per contatto con la pelle.
- H330-Letale se inalato.
- H314-Provoca gravi ustioni cutanee e gravi lesioni oculari.
- H315-Provoca irritazione cutanea.
- H319-Provoca grave irritazione oculare.
- H400-Molto tossico per gli organismi acquatici.
- EUH031-A contatto con acidi libera gas tossici.
- EUH208-Contiene . Può provocare una reazione allergica.
Precautionary Statement
- P280-Indossare guanti/indumenti protettivi/Proteggere gli occhi/il viso.
- P210-Tenere lontano da fonti di calore, superfici riscaldate, scintille, fiamme e altre fonti di innesco. Vietato fumare.
- P260-Non respirare la polvere/i fumi/i gas/la nebbia/i vapori/gli aerosol.
- P262-Evitare il contatto con gli occhi, la pelle o gli indumenti.
- P273-Non disperdere nell'ambiente.
- P235-Conservare in luogo fresco.
- P241-Utilizzare impianti elettrici/di ventilazione/d'illuminazione/ a prova di esplosione.
- P264-Lavare accuratamente dopo l'uso.
- P301+P310-IN CASO DI INGESTIONE: contattare immediatamente un CENTRO ANTIVELENI/un medico/
- P331-NON provocare il vomito.
- P303+P361+P353-IN CASO DI CONTATTO CON LA PELLE (o con i capelli): togliere immediatamente tutti gli indumenti contaminati. Sciacquare la pelle/fare una doccia.
- P304+P340-IN CASO DI INALAZIONE: trasportare l'infortunato all'aria aperta e mantenerlo a riposo in posizione che favorisca la respirazione.
- P305+P351+P338-IN CASO DI CONTATTO CON GLI OCCHI: sciacquare accuratamente per parecchi minuti. Togliere le eventuali lenti a contatto se è agevole farlo. Continuare a sciacquare.
- P310-Contattare immediatamente un CENTRO ANTIVELENI/un medico .
- P405-Conservare sotto chiave.
- P501-Smaltire il prodotto/recipiente in .
Supplemental information
- MIXTURE LIST-Contiene: sodium chloride, sodiumcyanotrihydroborate, sodium hydroxide, trometamol, sodiumchloride, Nonidet P
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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