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Thermo Scientific™ Kit Classic IP Pierce™
Perform traditional immunoprecipitation assays with ease using high-quality Protein A/G agarose beads, microcentrifuge spin columns and buffers.
Marca: Thermo Scientific™ 26146
302.68 EUR valido fino al 2025-06-21
Usa il codice promo "24112" per avere il tuo prezzo promozionale.
Allerta:
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24112” to get your promotional price
Descrizione
Thermo Scientific™ Pierce Classic IP Kit uses high-quality Protein A/G agarose beads and microcentrifuge spin columns to accomplish traditional immunoprecipitation assays with ease.
The Classic IP Kit provides all the necessary reagents, spin cups and collection tubes to easily perform successful immunoprecipitation (IP) experiments. The kit uses high-capacity Protein A/G agarose affinity resin for efficient binding of most species and subclasses of IP antibodies. The included IgG elution buffer provides milder and less denaturing recovery of antibody:antigen complexes than the traditional method of boiling in reducing sample buffer for SDS-PAGE, facilitating a greater variety of methods for subsequent analysis. The microcentrifuge spin column format helps to ensure effective washing and separation of samples from the beaded agarose affinity resin.Like traditional IP methods, the Pierce Classic IP protocol involves formation of antibody:antigen complexes in a sample solution and then capture of that complex to an IgG-binding protein that is covalently bound to beaded agarose resin (Protein A/G Agarose). After washing to remove nonbound (presumably undesired) components of the sample, the antigen and antibody are recovered from the beaded resin with elution buffer supplied in the kit. The entire procedure is performed in a microcentrifuge spin column, allowing solutions to be fully separated from the agarose resin upon brief centrifugation.
Highlights:
Assay consistency – spin columns eliminate resin loss, improve sample recovery and ensure efficient separation from resin
Easy protocol – immunoprecipitate (IP) target proteins from crude lysates in just four easy steps
Fast – complete an entire immunoprecipitation experiment in less than one hour
Economical – reuse the Protein A/G agarose resin for future immunoprecipitations
Complete – kit includes a compatible cell lysis reagent and sufficient components to perform at least 50 IP experiments
Includes:
Pierce Protein A/G Plus Agarose, lysis/wash buffer, conditioning buffer, Tris-buffered saline, elution buffer, lane marker sample buffer, control agarose resin, spin columns and collection tubes
Recommended for:
Routine, traditional immunoprecipitation experiments; Immunoprecipitation for analysis in nonreducing conditions
Specifica
| Sufficient For: At least 50 IP reactions with 10 μL resin • Protein A/G Plus Agarose, 0.55 mL • IP Lysis/Wash Buffer, 2 x 50 mL • Conditioning Buffer (100X), 5 mL • Tris-buffered Saline (20X), 25 mL • Elution Buffer, 50 mL • Lane Marker Sample Buffer (5X), 5 mL • Control Agarose Resin, 2 mL • Spin Columns and Collection Tubes Store at 4°C. |
|
| Microcentrifuge | |
| 50 reactions kit | |
| Not Target-Specific | |
| 50 Reactions |
| Affinity Purification | |
| Pierce™ | |
| Immunoprecipitation (IP) | |
| Immunoprecipitation/Co-Immunoprecipitation Kit | |
| Kit |
Sicurezza e movimentazione
Identificativo prodotto
Avvertenza
Categoria di pericolo
Indicazioni di pericolo
Consigli di prudenza
Informazioni supplementari
- Pierce Classic IP Kit
Avvertenza
- Attenzione
Categoria di pericolo
- Lesione oculare grave/irritazione oculare Categoria 2
- Liquido infiammabile Categoria 3
Indicazioni di pericolo
- H226-Liquido e vapori infiammabili.
- H319-Provoca grave irritazione oculare.
Consigli di prudenza
- P210-Tenere lontano da fonti di calore, superfici riscaldate, scintille, fiamme e altre fonti di innesco. Vietato fumare.
- P264-Lavare accuratamente dopo l'uso.
- P280-Indossare guanti/indumenti protettivi/Proteggere gli occhi/il viso.
- P303+P361+P353-IN CASO DI CONTATTO CON LA PELLE (o con i capelli): togliere immediatamente tutti gli indumenti contaminati. Sciacquare la pelle/fare una doccia.
- P305+P351+P338-IN CASO DI CONTATTO CON GLI OCCHI: sciacquare accuratamente per parecchi minuti. Togliere le eventuali lenti a contatto se è agevole farlo. Continuare a sciacquare.
- P337+P313-Se l'irritazione degli occhi persiste, consultare un medico.
- P370+P378-In caso d'incendio: utilizzare per estinguere.
- P403+P235-Conservare in luogo fresco e ben ventilato.
- P501b-Smaltire il prodotto/recipiente in conformità con le disposizioni locali/regionali/nazionali/internazionali.
Informazioni supplementari
- MIXTURE LIST-Contiene: Sodium chloride, ethanol
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Titolo del prodotto
For Research Use Only. Not for use in diagnostic procedures.
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