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Macherey-Nagel™ NucleoSpin™ RNA Plant, Mini kit for RNA from plant
Isolates total RNA from all kinds of plant material and filamentous fungi without degradation of the RNA. Macherey-Nagel™ NucleoSpin™ RNA Plant Kit is a complete mini spin kit that includes RNA plant columns with collection tubes, filters, all needed buffers and RNase-free rDNase.
Marca: Macherey-Nagel™ 740949.10
Descrizione
NucleoSpin™ RNA Plant Kit is designed for isolation of DNA-free, high-quality RNA from a wide variety of plant and fungal samples. Typical downstream applications include real-time RT-PCR, gene expression profiling, Northern blotting, primer extension, array technology, and RNase protection assays.
- Two different lysis buffers included—Optimized lysis procedure for typical conditions and for peculiar metabolites
- rDNase included for on-column digestion—Efficient removal of contaminating DNA
- NucleoSpin™ Filters (shredders) included—Efficient sample homogenization and reduction of viscosity
- Parallel purification of DNA possible—By using the NucleoSpin™ RNA/DNA buffer set
Kit Components: NucleoSpin™ RNA columns with 2 mL collection tubes, 2 mL and 1.5 mL collecting tubes, NucleoSpin™ filters, buffers (lysis, wash, desalting), RNase-free DNase I, reaction buffer for DNase, RNase-free water
- Mini spin column with silica membrane technology
- Yields 3 to 70μg RNA from 100μg plant material
- Elution volume: 40 to 60μL
- Fragment size: 200b–20kb
- Binding capacity: 200μg
- Preparation time: 30 min./6 preps
- A260/A280: 1.9–2.1
Specifica
Mini Spin Column | |
RNA purification from plant cells and tissue | |
10 Preps | |
1 Each | |
3–70μg |
Real-time RT-PCR | |
Lysis buffer RA1 (10mL), Lysis buffer RAP (10mL), Wash buffer RAW2 (13mL), Wash Buffer RA3 concentrate (6mL), Membrane desalting buffer (10mL), Reaction buffer for rDNase (7mL), RNase-free rDNase (1 size D vial), RNase-free water (13mL), NucleoSpin filters (10), NucleoSpin RNA Plan Columns with collection tubes (10), 2mL Collection tubes (30), 1.5mL Collection tubes (10) | |
NucleoSpin™ RNA Plant Column | |
<100 mg |
Sicurezza e movimentazione
- Pericolo
Categoria di pericolo
- Liquido infiammabile Categoria 3
- Tossicità acuta Categoria 4
- Corrosione/irritazione della pelle Categoria 2
- Lesione oculare grave/irritazione oculare Categoria 2
- Sensibilizzante delle vie respiratorie Categoria 1
- PERICOLO A LUNGO TERMINE PER L'AMBIENTE ACQUATICO Cronico 3
Indicazioni di pericolo
- H226-Liquido e vapori infiammabili.
- H302-Nocivo se ingerito.
- H315-Provoca irritazione cutanea.
- H319-Provoca grave irritazione oculare.
- H334-Può provocare sintomi allergici o asmatici o difficoltà respiratorie se inalato.
- H412-Nocivo per gli organismi acquatici con effetti di lunga durata.
Consigli di prudenza
- P261-Evitare di respirare la polvere/i fumi/i gas/la nebbia/i vapori/gli aerosol.
- P342+P311-In caso di sintomi respiratori: contattare un CENTRO ANTIVELENI/un medico/ .
Informazioni supplementari
- MIXTURE LIST-Contiene: ethanol (diluted < 20 %)(denatured with1%IPA/1%MEK, acc.2016/1867/EU); guanidinium thiocyanate; gua
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