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Macherey-Nagel™ NucleoSpin™ 96 PCR Clean-up, 96-well kit for PCR clean up
NucleoSpin™ 96 Gel and PCR Clean-Up Kit provides time saving medium to high throughput PCR clean up.
Marca: Macherey-Nagel™ 740658.24
Descrizione
The NucleoSpin™ 96 PCR Clean-up kit allows direct clean-up of PCR reaction mixtures. Within the procedure the addition of chaotropic salt (Buffer NT) allows a reversible adsorption of the PCR products to the silica membrane of the NucleoSpin™ 96 PCR Clean-up Binding Plates. High purity of the PCR-products is achieved by complete removal of primers, primer-dimers, salts, nucleotides, and proteins (e.g., polymerases, BSA) in subsequent washing steps using Buffer NT3. Highly pure PCR products are finally eluted with Elution Buffer NE (5 mM Tris/HCl, pH 8.5) or water (pH 8.5), and can be used directly for further applications.
- Silica membrane technology
- Processing: manual/automated, vacuum/centrifugation
- Sample material: < 10 0μL PCR reaction mixture
- Elution volume: 75 to 150 μL
- Binding capacity: 15 μg
- Preparation time: 45 min./plate
- Fragment size: 65 bp to 10 kb
Specifica
18°C to 25°C | |
PCR clean up | |
<100 μL |
96-well Plate | |
2304 Preps |
Sicurezza e movimentazione
- Attenzione
Categoria di pericolo
- Tossicità acuta Categoria 4
- PERICOLO A LUNGO TERMINE PER L'AMBIENTE ACQUATICO Cronico 3
Indicazioni di pericolo
- H302-Nocivo se ingerito.
- H412-Nocivo per gli organismi acquatici con effetti di lunga durata.
Informazioni supplementari
- MIXTURE LIST-Contiene: guanidinium thiocyanate
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