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Macherey-Nagel™ NucleoBond™ PC EF, kit for endotoxin-free plasmid DNA
Removes endotoxins via an additional washing step, without ice incubation. Macherey-Nagel™ NucleoBond™ PC EF Kit is a classic anion exchanger for purification of endotoxin-free plasmid DNA. The kit includes NucleoBond™ AX Columns, all buffers, RNase A and NucleoBond™ Filters.
Marca: Macherey-Nagel™ 740548
Descrizione
An important prerequisite for transfections and gene therapy applications is endotoxin-free plasmid DNA. The patented procedure of NucleoBond™ PC EF reduces endotoxins to a very low level using the same working procedure as the standard protocol. In comparison to competitive kits, there is no need for a time-consuming incubation step to remove the endotoxins.
Features
- Fast and easy clarification by NucleoBond™ Folded Filters—No centrifugation required, no shearing forces
- Proven anion-exchange technology—Yields transfection-grade high-copy plasmid DNA from E. coli
- NucleoBond™ Columns contain NucleoBond™ AX Silica Resin packed between inert filters
- Polypropylene columns are resistant to organic solvents and are free of DNase and RNase
- AX Resin can be used over a wide pH range and can remain in contact with buffers for up to three hours
Specifications
- DNA with less than 0.1 EU/μg DNA
- Purity of plasmid DNA: A260/A280 = 1.80–1.95
- Recovery of plasmid DNA >90%
- Structural integrity >90%
- Yield between 500μg up to 10mg, depending on kit used
Specifica
Endotoxin level: <0.1 EU/μg | |
Cromatografia a scambio anionico | |
High-copy plasmids from E. coli | |
5 | |
500 - 2 000 ml | |
Yes | |
<300 kbp | |
2,000–10,000μg |
Gravity-flow Column (Giga) | |
Recovery of plasmid DNA: >90% | |
NucleoBond AX 10000 Columns, buffers, RNase A, NucleoBond Bottle Top Filters | |
NucleoBond™ PC 10,000 EF | |
A260/A280: 1.80–1.95 | |
500 - 2 000 ml | |
180 min. |
Sicurezza e movimentazione
- Pericolo
Categoria di pericolo
- Liquido infiammabile Categoria 3
- Corrosione/irritazione della pelle Categoria 2
- Lesione oculare grave/irritazione oculare Categoria 2
- Sensibilizzante delle vie respiratorie Categoria 1
Indicazioni di pericolo
- H226-Liquido e vapori infiammabili.
- H315-Provoca irritazione cutanea.
- H319-Provoca grave irritazione oculare.
- H334-Può provocare sintomi allergici o asmatici o difficoltà respiratorie se inalato.
Consigli di prudenza
- P210-Tenere lontano da fonti di calore, superfici riscaldate, scintille, fiamme e altre fonti di innesco. Vietato fumare.
- P261-Evitare di respirare la polvere/i fumi/i gas/la nebbia/i vapori/gli aerosol.
- P342+P311-In caso di sintomi respiratori: contattare un CENTRO ANTIVELENI/un medico/ .
- P280-Indossare guanti/indumenti protettivi/Proteggere gli occhi/il viso.
Informazioni supplementari
- MIXTURE LIST-Contiene: ethanol (diluted < 20 %)/sodium hydroxide solution (diluted< 2 %)/dodecyl sulfate, sodium salt
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