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Macherey-Nagel™ NucleoBond™ PC EF, kit for endotoxin-free plasmid DNA
Removes endotoxins via an additional washing step, without ice incubation. Macherey-Nagel™ NucleoBond™ PC EF Kit is a classic anion exchanger for purification of endotoxin-free plasmid DNA. The kit includes NucleoBond™ AX Columns, all buffers, RNase A and NucleoBond™ Filters.
Marca: Macherey-Nagel™ 740549
Descrizione
An important prerequisite for transfections and gene therapy applications is endotoxin-free plasmid DNA. The patented procedure of NucleoBond™ PC EF reduces endotoxins to a very low level using the same working procedure as the standard protocol. In comparison to competitive kits, there is no need for a time-consuming incubation step to remove the endotoxins.
Features
- Fast and easy clarification by NucleoBond™ Folded Filters—No centrifugation required, no shearing forces
- Proven anion-exchange technology—Yields transfection-grade high-copy plasmid DNA from E. coli
- NucleoBond™ Columns contain NucleoBond™ AX Silica Resin packed between inert filters
- Polypropylene columns are resistant to organic solvents and are free of DNase and RNase
- AX Resin can be used over a wide pH range and can remain in contact with buffers for up to three hours
Specifications
- DNA with less than 0.1 EU/μg DNA
- Purity of plasmid DNA: A260/A280 = 1.80–1.95
- Recovery of plasmid DNA >90%
- Structural integrity >90%
- Yield between 500μg up to 10mg, depending on kit used
Specifica
Endotoxin level: <0.1 EU/μg | |
Anion Exchange Chromatography Technology | |
High-copy plasmids from E. coli | |
5 | |
1 Kit | |
Yes | |
<300 kbp | |
500–2,000μg |
Mega Gravity-flow Column | |
Recovery of plasmid DNA: >90% | |
NucleoBond AX 2000 Columns, buffers, RNase A, NucleoBond Bottle Top Filters | |
NucleoBond™ PC 2,000 EF | |
A260/A280: 1.80–1.95 | |
150 - 500mL | |
150 min./2 preps |
Sicurezza e movimentazione
- Liquido infiammabile Categoria 3
- Sostanze e miscele corrosive per i metalli Categoria 1
- Corrosione/irritazione della pelle Categoria 2
- Sensibilizzante delle vie respiratorie Categoria 1
- Lesione oculare grave/irritazione oculare Categoria 2
- Sensibilizzazione della pelle Categoria 1
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