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Macherey-Nagel™ NucleoBond™ PC 10000, Giga kit for transfection-grade plasmid DNA
1st generation anion exchanger for purification of plasmid DNA.
Marca: Macherey-Nagel™ 740593
Descrizione
NucleoBond™ kits utilize anion-exchange chromatography with optimized lysis buffer volumes for plasmid DNA purification. PC 10,000 kits are recommended for the isolation of high-copy plasmids (>20 copies/cell). Low-copy plasmids (<20 copies/cell) can be isolated as well, but this requires additional buffers.
- Folded Filters are included for fast and easy clarification of lysates with no centrifugation required and no shearing forces
- Contains sufficient buffer to perform 5 giga preps, with typical yields of 2,000–10,000μg of plasmid DNA from E. coli culture
- Columns are resistant to organic solvents and are free of DNase and RNase
- AX Resin can be used over a wide pH range (2.5–8.5) and can remain in contact with buffers for up to three hours
Specifica
Yes | |
Anion Exchange Chromatography Technology | |
Purification of high-copy and low-copy plasmids | |
5 Preps | |
1 Kit | |
500—2000mL (HC) | |
120–150 min/2 preps |
Gravity-flow Column (Giga) | |
E. coli Culture | |
Resuspension Buffer S1 (750mL), Lysis Buffer S2 (750mL), Neutralization Buffer S3 (750mL), Equilibration Buffer N2 (500/125mL), Wash Buffer N3 (1000/125mL), Elution Buffer N5 (500/125mL), RNase A lyophilized (75mg), NucleoBond AX 10000 Columns (5), NucleoBond Folded Filters Type 1 (10), NucleoBond Folded Filters Type 2 (10) | |
PC 10000 Columns | |
A260/A280: 1.80–1.95 | |
<300 kbp | |
2,000–10,000μg |
Sicurezza e movimentazione
- Liquido infiammabile Categoria 3
- Sostanze e miscele corrosive per i metalli Categoria 1
- Corrosione/irritazione della pelle Categoria 2
- Sensibilizzante delle vie respiratorie Categoria 1
- Lesione oculare grave/irritazione oculare Categoria 2
- Sensibilizzazione della pelle Categoria 1
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