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  7. Invitrogen™ Taq DNA Polymerase, recombinant

Invitrogen™ Taq DNA Polymerase, recombinant

Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.

Specifica

Formato Stand-alone enzyme
GC-Rich PCR Performance Low
PCR Method qPCR, Standard PCR
Polimerasi Taq DNA Polymerase
Reaction Speed Standard
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Includes: Taq DNA Polymerase (5U/μL); 10X PCR buffer (200mM Tris-HCl pH 8.4, 500mM KCl); Magnesium Chloride (50mM)

Descrizione

Descrizione

Taq DNA Polymerase, recombinant, is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. This recombinant enzyme is purified from the cloned Thermus aquaticus DNA polymerase gene expressed in E. coli and consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5' to 3' DNA polymerase activity and a 5' to 3' exonuclease activity.

Features

  • A choice of recombinant or native enzyme
  • Amplification of PCR products up to 5 kb in size
  • Enzyme is licensed and qualified for PCR

Applications

  • Amplification of DNA from complex genomic, viral, and plasmid templates
  • RT-PCR
  • Sequencing ssDNA
  • Cycle sequencing

Notes

  • One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 minutes at 74°C.
  • For superior PCR performance, DreamTaq DNA Polymerase is recommended.

Order Info

Shipping Condition: Approved for shipment on wet or dry ice

Specifica

Specifica

Stand-alone enzyme
qPCR, Standard PCR
Standard
DNA Polymerase
Standard PCR
1X
3'-A
5 kb or less
Low
Taq DNA Polymerase
5' - 3'
Dry Ice
5 U/μL
No
Separate Components
DNA
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SDS
Documenti

Documenti

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

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