Learn More
Thermo Scientific™ HisPur™ Cobalt Purification Kit, 3 mL
Gently purify His-tagged recombinant fusion proteins using cobalt (Co2+) immobilized metal chelate affinity (IMAC) beads, spin columns and plates.
Marca: Thermo Scientific™ 90092
318.68 EUR valido fino al 2025-06-21
Usa il codice promo "24112" per avere il tuo prezzo promozionale.
Allerta:
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24112” to get your promotional price
Descrizione
Thermo Scientific™ HisPur Cobalt Resin is an immobilized metal chelate affinity (IMAC) support for specific, fast and gentle His-tagged purification.
Additional Information and Online Ordering
HisPur Cobalt Resins, Columns and Kits use a tetradentate chelating resin charged with divalent cobalt (Co2+)to enable specific, fast and gentle purification of histidine-tagged fusion proteins from a variety of sample types. HisPur Cobalt Resin is available in several package sizes of resin slurry, three sizes of centrifuge-ready columns, complete purification kits, two sizes of FPLC-ready chromatography cartridges, and 96-well filter plates for high throughput needs. With these options, high-quality His-tag protein purification is possible at nearly any scale. HisPur Cobalt Resin is superior to traditional nickel-based immobilized metal affinity chromatography (IMAC) systems. Compared to Ni-IDA and tetradentate Ni2+chelate resins, HisPur Resin binds histidine-tagged proteins with higher specificity (less off-target binding) and releases them with gentler conditions (lower concentrations of imidazole). Use HisPur Columns and Cartridges to obtain high-purity His-tagged proteins with no metal contamination.Highlights:
High purity – obtain more than 10 milligrams of pure His-tagged protein per milliliter of resin without optimizing imidazole washing conditions
Low metal leaching – no metal contamination in eluted histidine-tagged protein sample
Versatility – purify proteins under native or denaturing conditions; compatible with cell lysis reagents and a variety of buffer additives
Flexibility – available as bulk resin or predispensed columns, chromatography columns and 96-well filter plates
Cost effective – resin can be reused several times or discarded after single use
Superior – performs better than other commercially available IMAC Resins
Available Formats:
- Resin Slurries – crosslinked 6% beaded agarose; 10mL, 100mL, 500mL bottles
- Spin Columns – 0.2mL (microcentrifuge), 1mL, 3mL columns
- Purification Kits – complete kits in all three column sizes
- Chromatography Cartridges – compatible with automated liquid chromatography systems and with syringe processing
- 96-well Spin Plates – pre-dispensed 96-well filter plates, compatible with centrifugation and vacuum manifold systems for manual or automated purification. The plates enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput separations
- Bulk and Custom – let us know your needs for bulk HisPur Cobalt Resin
Includes:
Kits contain HisPur Cobalt Columns, equilibration/wash buffer and elution buffer

Specifica
1 mL/Column | |
Sufficient For: Purifying ∼30 mg of His-tagged protein per column • HisPur Cobalt Spin Columns, 3 mL, 5 columns • Equilibration/Wash Buffer, 225 mL • Elution Buffer, 75 mL Store at 4°C. |
|
5 Column Kit | |
Protein Purification System | |
2 mg of His-Tagged Protein per column | |
Affinity, Agarose Resin |
HisPur Cobalt Spin Purification Kit, 3mL | |
HisPur™ | |
His-Tagged Protein | |
Metal Chelate, Divalent Cobalt | |
Spin Column |
Sicurezza e movimentazione
- HisPur Cobalt Purification Kit, 3 mL
Avvertenza
- Pericolo
Categoria di pericolo
- Lesione oculare grave/irritazione oculare Categoria 2
- Liquido infiammabile Categoria 3
- Tossicità per la riproduzione Categoria 1B
Indicazioni di pericolo
- H226-Liquido e vapori infiammabili.
- H319-Provoca grave irritazione oculare.
- H360-Può nuocere alla fertilità o al feto.
Consigli di prudenza
- P201-Procurarsi istruzioni specifiche prima dell'uso.
- P202-Non manipolare prima di avere letto e compreso tutte le avvertenze.
- P210-Tenere lontano da fonti di calore, superfici riscaldate, scintille, fiamme e altre fonti di innesco. Vietato fumare.
- P241-Utilizzare impianti elettrici/di ventilazione/d'illuminazione/ a prova di esplosione.
- P264-Lavare accuratamente dopo l'uso.
- P280-Indossare guanti/indumenti protettivi/Proteggere gli occhi/il viso.
- P305+P351+P338-IN CASO DI CONTATTO CON GLI OCCHI: sciacquare accuratamente per parecchi minuti. Togliere le eventuali lenti a contatto se è agevole farlo. Continuare a sciacquare.
- P308+P313-IN CASO di esposizione o di possibile esposizione, consultare un medico.
- P337+P313-Se l'irritazione degli occhi persiste, consultare un medico.
- P403+P235-Conservare in luogo fresco e ben ventilato.
- P501b-Smaltire il prodotto/recipiente in conformità con le disposizioni locali/regionali/nazionali/internazionali.
Informazioni supplementari
- MIXTURE LIST-Contiene: Ethanol, 1-Imidazole, Sodium chloride
Fornite il vostro feedback sul contenuto del prodotto compilando il modulo sottostante.
For Research Use Only. Not for use in diagnostic procedures.