Cytiva HiPrep™ Sephacryl™ HR Prepacked Gel Filtration Columns

Aids in preparative separation of biomolecules from up to 5mL (for 16mm column I.D.) and 13mL (for 26mm column I.D.).

• Provides maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability
• Prepacked columns for use with AKTAdesign™ or other pumped chromatography systems
• Available in two different prepacked column sizes - 16 x 600mm (120mL) and 26 x 60mm (320mL)
• Maximum pressure (over packed bed during operation) is 1.5 bar (22psi)

Media    

• Average particle size: 50μm
• Matrix: Allyl dextran and N, N'-methylenebisacrylamide
• pH Stability working range: 3 to 11
• pH Stability cleaning range: 2 to 13
• Chemical stability: all commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1% SDS, 20% ethanol, 30% propanol, 30% acetonitrile (tested at 40°C for one week), 0.5 M NaOH (only for cleaning-in-place)
• Pressure/Flow specification: >125cm/hr., 100kPa, XK 50/30, bed height 15cm

• Material (bed support): Polymethyl-Methacrylate

HiPrep 16/60 Sephacryl S-400 HR

• Media:
• Fractionation [Mr] globular proteins: 2 x 104^-8 x 106
• Exclusion limit [Mr] (globular proteins): ≈9 x 106 (9000kDa)
• Fractionation range [Mr] DNA exclusion limit:    271bp
• Fractionation [Mr] Dextrans: 1 x 104^-2 x 106

• Media:
• Exclusion limit for Dextrans: 100 x 106
• Fractionation range [Mr] DNA exclusion limit: 1078bp
• Fractionation [Mr] Dextrans: 4 x 104^-2 x 107