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Cytiva Amersham™ AlkPhos Direct™ ECF Detection Module
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AlkPhos Direct Labeling and Detection Systems are based on the rapid, direct labeling of DNA or RNA probes with thermostable alkaline phosphatase. CDP-Star™ or ECF™ detection can be used. DNA or RNA probes are labeled during a 30-minute reaction and resulting probes can be used without purification. Hybridization stringency can be controlled by temperature as well as salt concentration.
- Hybridization stringency can be controlled by elevating temperatures up to 75°C
- Detection of 60 fg target DNA (single-copy gene) in a genomic Southern blot
- ECF detection for quantitation experiments
- Rapid protocol saves two to three hours over conventional indirect methods
Order Info
Each system include the following reagents, sufficient for 25 or 50 labeling reactions and detection of 2500cm2: labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer, blocking agent, ECF detection reagents.
Specifica
Specifica
| Sufficiente per | 2500cm2 membrane |
| Include | Sufficient for 25 or 50 labeling reactions and detection of 2500cm2 membrane: labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer, blocking agent, ECF detection reagents. |
| Quantità | Each |
Sicurezza e movimentazione
- Pericolo
- Lesione oculare grave/irritazione oculare Categoria 1
- Corrosione/irritazione della pelle Categoria 2
- Tossicità specifica per organi bersaglio (esposizione ripetuta) Categoria 2
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