Thermo Scientific™ PscI (PciI)

Descrizione

Lambda DNA digested with PscI (PciI), 0.7% agarose, 2 cleavage sites

conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

5'...A▵C A T G T...3'

3'...T G T A C▵A...5'

Conditions for 100% Activity

• 1X Buffer Tango:33mM Tris-acetate (pH7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
• Incubate at 37°C

Storage Buffer

• PscI is supplied in:10mM Tris-HCl (pH7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage

• After 50-fold overdigestion with PscI, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects

• Dam: never overlaps —no effect
• Dcm: never overlaps —no effect
• CpG: never overlaps —no effect
• EcoKI: may overlap —effect not determined
• EcoBI: may overlap —effect not determined

Digestion of Agarose-embedded DNA

• Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours

Compatible Ends

• AflIII, BtgI, Eco130I, FatI, NcoI, PagI

Note:

PscI, like its isoschizomers BspLU11I and PciI, is inhibited by nonionic detergents Triton X-100 (>0.002%) and Nonidet P40 (>0.001%).