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Cytiva bacteria genomicPrep Mini Spin Kit
Designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from Gram-negative (G-) and Gram-positive (G+) bacteria
267.00€ - 1151.00€
Specifica
Descrizione | illustra bacteria genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from Gram-negative (G-) and Gram-positive (G+) bacteria. |
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Da utilizzare con (applicazione) | For the rapid extraction and purification of high-quality genomic DNA from gram-positive and gram-negative bacteriaPurified gDNA is suitable for molecular biology applications including cloning, restriction enzyme digestion, PCR and genotyping |
Product Type | illustra bacteria genomicPrep Mini Spin Kit |
Purezza | 1.7 |
Volume del campione | 2 x 109 cells [A600 = 2.0] |
Codice del prodotto | Marca | N. di reazioni | Prezzo | Quantità e disponibilità | |||||
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Codice del prodotto | Marca | N. di reazioni | Prezzo | Quantità e disponibilità | |||||
10536485
|
Cytiva
28-9042-58 |
50 Preps |
267.00€
Pezzo |
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10546485
|
Cytiva
28-9042-59 |
250 Preps |
1151.00€
250 reazioni |
Effettua il login per acquistare questo articolo Hai bisogno di un conto web ? Registrati oggi stesso! | |||||
Descrizione
The illustra Bacteria genomicPrep Mini Spin Kit can be used with a wide range of bacterial strains such as DH5a, TOP10, JM109, Bacillus subtilis etc. The kit produces highly pure and intact genomic DNA with OD260/280 values of 1.75-1.85. Use different elution volumes to isolate genomic DNA at concentrations suitable for most downstream applications including restriction enzyme digestion, RT-PCR, and long PCR.
The method uses a lysis solution in combination with proteinase K to gently release genomic DNA into solution from bacteria cells. The DNA is deproteinated in the extraction solution and the genomic DNA is then bound onto a silica column in the presence of chaotropic solution. Contaminants are removed during wash steps and the purifed DNA is eluted with prewarmed TE solution.
- Total DNA extraction time under one hour with reduced lysis time
- Can be used to purify 4 to 8μg of genomic DNA from 2 × 109 bacteria cells with input amounts ranging from 1 × 109 to 4 × 109 bacteria cells
- Spin columns prepacked with a silica membrane—provide excellent reproducibility and consistency
- Validated in several downstream applications including RT PCR, long PCR, and restriction digest
- Color-coded caps for ease of use
Specifica
illustra bacteria genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from Gram-negative (G-) and Gram-positive (G+) bacteria. | |
illustra bacteria genomicPrep Mini Spin Kit | |
2 x 109 cells [A600 = 2.0] |
For the rapid extraction and purification of high-quality genomic DNA from gram-positive and gram-negative bacteriaPurified gDNA is suitable for molecular biology applications including cloning, restriction enzyme digestion, PCR and genotyping | |
1.7 | |
40 min [Gram-negaitive Bacteria], 55 min [Gram-positive Bacteria] |
Sicurezza e movimentazione
- Pericolo
Categoria di pericolo
- Lesione oculare grave/irritazione oculare Categoria 2
- Corrosione/irritazione della pelle Categoria 2
- Sensibilizzazione della pelle Categoria 1
- Tossicità specifica per organi bersaglio (esposizione singola) Categoria 3
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