missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Cytiva bacteria genomicPrep Mini Spin Kit
Designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from Gram-negative (G-) and Gram-positive (G+) bacteria
2559.36 SEK - 10819.00 SEK
Specifications
| Description | illustra bacteria genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from Gram-negative (G-) and Gram-positive (G+) bacteria. |
|---|---|
| For Use With (Application) | For the rapid extraction and purification of high-quality genomic DNA from gram-positive and gram-negative bacteriaPurified gDNA is suitable for molecular biology applications including cloning, restriction enzyme digestion, PCR and genotyping |
| Product Type | illustra bacteria genomicPrep Mini Spin Kit |
| Purity | 1.7 |
| Sample Volume | 2 x 109 cells [A600 = 2.0] |
| Product Code | Brand | No. of Reactions | Price | Quantity & Availability | |||||
|---|---|---|---|---|---|---|---|---|---|
| Product Code | Brand | No. of Reactions | Price | Quantity & Availability | |||||
|
10536485
|
Cytiva
28-9042-58 |
50 Preps |
2559.36 SEK
Each |
Please sign in to purchase this item. Need a web account? Register with us today! | |||||
|
10546485
|
Cytiva
28-9042-59 |
250 Preps |
10819.00 SEK
250 reactions |
Please sign in to purchase this item. Need a web account? Register with us today! | |||||
Description
The illustra Bacteria genomicPrep Mini Spin Kit can be used with a wide range of bacterial strains such as DH5a, TOP10, JM109, Bacillus subtilis etc. The kit produces highly pure and intact genomic DNA with OD260/280 values of 1.75-1.85. Use different elution volumes to isolate genomic DNA at concentrations suitable for most downstream applications including restriction enzyme digestion, RT-PCR, and long PCR.
The method uses a lysis solution in combination with proteinase K to gently release genomic DNA into solution from bacteria cells. The DNA is deproteinated in the extraction solution and the genomic DNA is then bound onto a silica column in the presence of chaotropic solution. Contaminants are removed during wash steps and the purifed DNA is eluted with prewarmed TE solution.
- Total DNA extraction time under one hour with reduced lysis time
- Can be used to purify 4 to 8μg of genomic DNA from 2 × 109 bacteria cells with input amounts ranging from 1 × 109 to 4 × 109 bacteria cells
- Spin columns prepacked with a silica membrane—provide excellent reproducibility and consistency
- Validated in several downstream applications including RT PCR, long PCR, and restriction digest
- Color-coded caps for ease of use
Specifications
| illustra bacteria genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from Gram-negative (G-) and Gram-positive (G+) bacteria. | |
| illustra bacteria genomicPrep Mini Spin Kit | |
| 2 x 109 cells [A600 = 2.0] |
| For the rapid extraction and purification of high-quality genomic DNA from gram-positive and gram-negative bacteriaPurified gDNA is suitable for molecular biology applications including cloning, restriction enzyme digestion, PCR and genotyping | |
| 1.7 | |
| 40 min [Gram-negaitive Bacteria], 55 min [Gram-positive Bacteria] |
Safety and Handling
Signal Word
Hazard Category
- Danger
Hazard Category
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- Skin sensitiser Category 1
- Specific target organ toxicity Category 3
Spot an opportunity for improvement?Share a Content Correction
Product Content Correction
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
Product Title