Applied Biosystems™ TaqMan™ Reverse Transcription Reagents

Descrizione

TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)₁₆, and reverse primers are included for cDNA synthesis flexibility. These reagents are designed for reverse transcription and PCR amplification of a specific target RNA from either total RNA or mRNA. The reagents in this kit facilitate two-step RT-PCR, which is performed in two separate reactions and is useful when detecting multiple transcripts from a single cDNA reaction or when storing a portion of the cDNA.

Advantages of TaqMan Reverse Transcription Reagents

• Contains MultiScribe Reverse Transcriptase, a recombinant Moloney murine leukemia virus (MMLV) reverse transcriptase
• Includes random hexamers and oligo (dT)₁₆ for flexibility
• Individual components provide versatility in assay setup
• Compatible with two-step RT-PCR reactions

Using TaqMan Reverse Transcription Reagents

• TaqMan Reverse Transcription Reagents provide all the necessary components for reverse transcription of RNA to cDNA
• For two-step RT-PCR, random hexamers, oligo (dT)₁₆, and sequence-specific reverse primers can all be used
• The choice of primers for reverse transcription is best made after experimentally evaluating all three priming systems