Invitrogen™ TURBO DNase™ (2 U/μL)

Descrizione

Cleaves double-stranded DNA nonspecifically to leave 5' phosphorylated oligodeoxynucleotides with an increased affinity for DNA-binding and remains active in presence of salt.

• Up to 50X more activity and 350% greater catalytic efficiency
• Efficiently degrades DNA in solutions containing up to 0.25M salt
• Efficiently digests DNA to oligonucleotides
• Used in clearing DNA templates from in vitro transcription reactions
• RNase-free and recombinant in origin

• Used to clear DNA contamination from RNA samples prior to RT-PCR
• Conventional DNase I has a poor affinity for DNA and cleaves DNA of low concentration
• Salt-sensitive; 20 mM NaCl can reduce activity of enzyme by 30%
• Purified from bovine pancreas, one of richest natural sources of RNase A
• Threat of contaminating RNase activity in DNase I preparations requires that enzyme be exhaustively purified

TURBO DNA-free (Mfr. No. AM1907) is available. It contains TURBO DNase along with reagents to inactivate the enzyme and remove divalent cations from the sample post-digestion.

PCR and Real-Time PCR, Real Time PCR (qPCR), Reverse Transcription

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