Antibotics, Buffers and Supplements

Gibco™ Trypsin-EDTA (0.05%), phenol red

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas

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Gibco™ 2-Mercaptoethanol

A potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals

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Gibco™ Trypan Blue Solution, 0.4%

Routinely used as a cell stain to assess cell viability using the dye exclusion test

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Gibco™ Trypsin-EDTA (0.25%), phenol red

Made from trypsin powder, irradiated mixture of proteases derived from porcine pancreas

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Corning™ Matrigel™ hESC-Qualified Matrix

Compatible with mTeSR™1-devloped by StemCell Technologies. Corning™ Matrigel™ hESC-Qualified Matrix is widely accepted as an alternative substrate for the culture of hES cells as well as human induced pluripotent stem (iPS) cells.

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Corning™ Matrigel™ GFR Membrane Matrix

Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ GFR Basement Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.

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Gibco™ Trypsin-EDTA (0.5%), no phenol red

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas

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Gibco™ TrypLE™ Express Enzyme (1X), no phenol red

Unlike porcine trypsin, free of animal-derived components

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Gibco™ MEM Non-Essential Amino Acids Solution (100X)

Used as a growth supplement for cell culture medium, to increase cell growth and viability

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Corning™ Fibronectin, Human

Promotes cellular attachment, spreading, and proliferation on tissue-culture surfaces. Corning™ Fibronectin, Human can be used as an additive to serum-free culture media.

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Gibco™ Bacto™ Tryptone

For use with dehydrated media culture

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Corning™ MITO+ Serum Extender

Used to culture a variety of cells under serum-free or serum-reduced conditions

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Gibco™ Pluronic™ F-68 Non-ionic Surfactant (100X)

A non-ionic surfactant used to control shear forces in suspension cultures

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Corning™ BioCoat™ Poly-D-Lysine

Recommended for the culture of neurons, glial cells, and transfected cells

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Gibco™ Bacto™ Yeast Extract

Formulation of culture media for cultivation of heterotrophic microorganisms. Gibco™ Bacto™ Dehydrated Culture Media Additive: Yeast Extract is supplied in dehydrated form for the convenience of the laboratorian and bioprocessing specialist.

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HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents

CAS: 7365-45-9 Molecular Formula: C8H17N2NaO4S Molecular Weight (g/mol): 260.28 MDL Number: MFCD00006158 InChI Key: RDZTWEVXRGYCFV-UHFFFAOYSA-M Synonym: N-(2-Hydroxyethyl)piperazine-N«-2-ethanesulfonic Acid PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: [Na+].OCCN1CCN(CCS([O-])(=O)=O)CC1

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Gibco™ L-Glutamine (200 mM)

Ready-to-use stock solution that acts as a cell culture supplement

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Gibco™ Gurr Buffer Tablets

A convenient way to prepare a pH 6.8 phosphate buffer for dilution of Giemsa stain

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Gibco™ Insulin-Transferrin-Selenium (ITS -G) (100X)

A basal medium supplement to reduce the amount of fetal bovine serum (FBS) needed to culture cells

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Gibco™ Geltrex™ hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix

Geltrex™ hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix

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Corning™ Trypan Blue

Can be used for variety cell counting methods requiring information on cell count and viability.

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Merck Millipore Polybrene Infection/Transfection Reagent

A highly efficient method of gene transfer into mammalian cells leveraging infection with retroviral vectors.

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HEPES Free Acid, >99%, Molecular Biology Reagent, MP Biomedicals™

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 260.28 MDL Number: MFCD00006158 InChI Key: RDZTWEVXRGYCFV-UHFFFAOYSA-M Synonym: N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: sodium 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonate SMILES: [Na+].OCCN1CCN(CCS([O-])(=O)=O)CC1

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